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35-μm cell strainer snap cap on a flow cytometry tube  (Corning Life Sciences)

 
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    Corning Life Sciences 35-μm cell strainer snap cap on a flow cytometry tube
    F-ATPase/Ca v 2.3 functional complexes regulate ERK 1/2 phosphorylation and ROS production after fMLP activation of neutrophils. a. A representative western blot is presented to show the effect of F-ATPase/Ca v 2.3 functional complexes on ERK 1/2 phosphorylation. Neutrophils were pretreated with 400 nM SNX482 and 50 μg/ml oligomycin A in Ca 2+ -containing HBSS for 30 min, followed by 1 min of 100 nM fMLP activation. Cells in Ca 2+ -containing HBSS or Ca 2+ -free HBSS incubated with or without fMLP were used as positive or negative controls, respectively. b. The effect of F-ATPase/Ca v 2.3 functional complexes on ROS production was assessed by flow <t>cytometry.</t> DHR123-loaded neutrophils were pretreated with 400 nM SNX482 and 50 μg/ml oligomycin A in Ca 2+ -containing HBSS for 30 min, followed by 30 min of 100 nM fMLP incubation. Cells in Ca 2+ -containing HBSS or Ca 2+ -free HBSS incubated with or without fMLP were used as positive or negative controls, respectively
    35 μm Cell Strainer Snap Cap On A Flow Cytometry Tube, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/35-%CE%BCm+cell+strainer+snap+cap+on+a+flow+cytometry+tube/cell+strainer+35+%CE%BCm/pmc07001235-143-16-19
    Average 90 stars, based on 1 article reviews
    35-μm cell strainer snap cap on a flow cytometry tube - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "F0F1 ATP synthase regulates extracellular calcium influx in human neutrophils by interacting with Ca v 2.3 and modulates neutrophil accumulation in the lipopolysaccharide-challenged lung"

    Article Title: F0F1 ATP synthase regulates extracellular calcium influx in human neutrophils by interacting with Ca v 2.3 and modulates neutrophil accumulation in the lipopolysaccharide-challenged lung

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-020-0515-3

    F-ATPase/Ca v 2.3 functional complexes regulate ERK 1/2 phosphorylation and ROS production after fMLP activation of neutrophils. a. A representative western blot is presented to show the effect of F-ATPase/Ca v 2.3 functional complexes on ERK 1/2 phosphorylation. Neutrophils were pretreated with 400 nM SNX482 and 50 μg/ml oligomycin A in Ca 2+ -containing HBSS for 30 min, followed by 1 min of 100 nM fMLP activation. Cells in Ca 2+ -containing HBSS or Ca 2+ -free HBSS incubated with or without fMLP were used as positive or negative controls, respectively. b. The effect of F-ATPase/Ca v 2.3 functional complexes on ROS production was assessed by flow cytometry. DHR123-loaded neutrophils were pretreated with 400 nM SNX482 and 50 μg/ml oligomycin A in Ca 2+ -containing HBSS for 30 min, followed by 30 min of 100 nM fMLP incubation. Cells in Ca 2+ -containing HBSS or Ca 2+ -free HBSS incubated with or without fMLP were used as positive or negative controls, respectively
    Figure Legend Snippet: F-ATPase/Ca v 2.3 functional complexes regulate ERK 1/2 phosphorylation and ROS production after fMLP activation of neutrophils. a. A representative western blot is presented to show the effect of F-ATPase/Ca v 2.3 functional complexes on ERK 1/2 phosphorylation. Neutrophils were pretreated with 400 nM SNX482 and 50 μg/ml oligomycin A in Ca 2+ -containing HBSS for 30 min, followed by 1 min of 100 nM fMLP activation. Cells in Ca 2+ -containing HBSS or Ca 2+ -free HBSS incubated with or without fMLP were used as positive or negative controls, respectively. b. The effect of F-ATPase/Ca v 2.3 functional complexes on ROS production was assessed by flow cytometry. DHR123-loaded neutrophils were pretreated with 400 nM SNX482 and 50 μg/ml oligomycin A in Ca 2+ -containing HBSS for 30 min, followed by 30 min of 100 nM fMLP incubation. Cells in Ca 2+ -containing HBSS or Ca 2+ -free HBSS incubated with or without fMLP were used as positive or negative controls, respectively

    Techniques Used: Functional Assay, Activation Assay, Western Blot, Incubation, Flow Cytometry

    Related Articles

    Filtration:

    Article Title: F0F1 ATP synthase regulates extracellular calcium influx in human neutrophils by interacting with Ca v 2.3 and modulates neutrophil accumulation in the lipopolysaccharide-challenged lung
    Article Snippet: .. The aggregated cells were removed by filtration through a 35-μm cell strainer snap cap on a flow cytometry tube (Falcon, Corning, Cambridge, MA). .. Then, neutrophils were recognized via a BD Accuri C6 Flow Cytometer (BD Biosciences, San Jose, CA, USA) on the basis of forward light scatter (FSC) and side light scatter (SSC), which identified neutrophils and excluded other cell types (a few contaminating lymphocytes and red blood cells), dead cells and debris from the analysis.

    Flow Cytometry:

    Article Title: F0F1 ATP synthase regulates extracellular calcium influx in human neutrophils by interacting with Ca v 2.3 and modulates neutrophil accumulation in the lipopolysaccharide-challenged lung
    Article Snippet: .. The aggregated cells were removed by filtration through a 35-μm cell strainer snap cap on a flow cytometry tube (Falcon, Corning, Cambridge, MA). .. Then, neutrophils were recognized via a BD Accuri C6 Flow Cytometer (BD Biosciences, San Jose, CA, USA) on the basis of forward light scatter (FSC) and side light scatter (SSC), which identified neutrophils and excluded other cell types (a few contaminating lymphocytes and red blood cells), dead cells and debris from the analysis.



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    35-μm cell strainer snap cap on a flow cytometry tube  (Corning Life Sciences)
    90
    Corning Life Sciences 35-μm cell strainer snap cap on a flow cytometry tube
    F-ATPase/Ca v 2.3 functional complexes regulate ERK 1/2 phosphorylation and ROS production after fMLP activation of neutrophils. a. A representative western blot is presented to show the effect of F-ATPase/Ca v 2.3 functional complexes on ERK 1/2 phosphorylation. Neutrophils were pretreated with 400 nM SNX482 and 50 μg/ml oligomycin A in Ca 2+ -containing HBSS for 30 min, followed by 1 min of 100 nM fMLP activation. Cells in Ca 2+ -containing HBSS or Ca 2+ -free HBSS incubated with or without fMLP were used as positive or negative controls, respectively. b. The effect of F-ATPase/Ca v 2.3 functional complexes on ROS production was assessed by flow <t>cytometry.</t> DHR123-loaded neutrophils were pretreated with 400 nM SNX482 and 50 μg/ml oligomycin A in Ca 2+ -containing HBSS for 30 min, followed by 30 min of 100 nM fMLP incubation. Cells in Ca 2+ -containing HBSS or Ca 2+ -free HBSS incubated with or without fMLP were used as positive or negative controls, respectively
    35 μm Cell Strainer Snap Cap On A Flow Cytometry Tube, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/35-%CE%BCm+cell+strainer+snap+cap+on+a+flow+cytometry+tube/cell+strainer+35+%CE%BCm/pmc07001235-143-16-19
    Average 90 stars, based on 1 article reviews
    35-μm cell strainer snap cap on a flow cytometry tube - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    F-ATPase/Ca v 2.3 functional complexes regulate ERK 1/2 phosphorylation and ROS production after fMLP activation of neutrophils. a. A representative western blot is presented to show the effect of F-ATPase/Ca v 2.3 functional complexes on ERK 1/2 phosphorylation. Neutrophils were pretreated with 400 nM SNX482 and 50 μg/ml oligomycin A in Ca 2+ -containing HBSS for 30 min, followed by 1 min of 100 nM fMLP activation. Cells in Ca 2+ -containing HBSS or Ca 2+ -free HBSS incubated with or without fMLP were used as positive or negative controls, respectively. b. The effect of F-ATPase/Ca v 2.3 functional complexes on ROS production was assessed by flow cytometry. DHR123-loaded neutrophils were pretreated with 400 nM SNX482 and 50 μg/ml oligomycin A in Ca 2+ -containing HBSS for 30 min, followed by 30 min of 100 nM fMLP incubation. Cells in Ca 2+ -containing HBSS or Ca 2+ -free HBSS incubated with or without fMLP were used as positive or negative controls, respectively

    Journal: Cell Communication and Signaling : CCS

    Article Title: F0F1 ATP synthase regulates extracellular calcium influx in human neutrophils by interacting with Ca v 2.3 and modulates neutrophil accumulation in the lipopolysaccharide-challenged lung

    doi: 10.1186/s12964-020-0515-3

    Figure Lengend Snippet: F-ATPase/Ca v 2.3 functional complexes regulate ERK 1/2 phosphorylation and ROS production after fMLP activation of neutrophils. a. A representative western blot is presented to show the effect of F-ATPase/Ca v 2.3 functional complexes on ERK 1/2 phosphorylation. Neutrophils were pretreated with 400 nM SNX482 and 50 μg/ml oligomycin A in Ca 2+ -containing HBSS for 30 min, followed by 1 min of 100 nM fMLP activation. Cells in Ca 2+ -containing HBSS or Ca 2+ -free HBSS incubated with or without fMLP were used as positive or negative controls, respectively. b. The effect of F-ATPase/Ca v 2.3 functional complexes on ROS production was assessed by flow cytometry. DHR123-loaded neutrophils were pretreated with 400 nM SNX482 and 50 μg/ml oligomycin A in Ca 2+ -containing HBSS for 30 min, followed by 30 min of 100 nM fMLP incubation. Cells in Ca 2+ -containing HBSS or Ca 2+ -free HBSS incubated with or without fMLP were used as positive or negative controls, respectively

    Article Snippet: The aggregated cells were removed by filtration through a 35-μm cell strainer snap cap on a flow cytometry tube (Falcon, Corning, Cambridge, MA).

    Techniques: Functional Assay, Activation Assay, Western Blot, Incubation, Flow Cytometry